אַל: not, no, neither
particle: adverb

la; + the prefix form of the verb expresses a negative command.

שׁלח: send
verb, qal, prefix, second person masculine singular

The prefix pronoun T can be either 3 f. sg. or 2 m. sg. Context will tell you which is meant.

עשׂה: do be done be done make be made be made made cause work do assign gain
verb, qal, prefix, second person masculine singular

Here we have another negative command. That means that the form of the verb is prefix. The T then is the prefix pronoun and not part of the root. The root is found using the 3^rd h missing letter rule.

עַתָּה: now so
particle: adverb

Do not confuse this adverb with the independent subject pronoun hT'a;

ידע: know
verb, qal, affix, first person common singular

This verb uses the affix form although present tense may give a better English translation.

יָרֵא: fear
adjective, masculine singular construct

This is an adjective in the construct form and means “fearing, reverencing”. Because it is masculine and singular, it can imply “one who fears” or “a fearer”.

אַתָּה: you
pronoun, second person masculine singular

מִן: from
particle: preposition with first person common singular suffix

When the preposition !mi appears with a suffix it takes this lengthened form.

אַחַר: after
particle: adverb

This can be the preposition after, behind as in Gen.22:1 or the adverb hind part, back part.

אחז: grasp, take hold, take possession
verb, niphal, affix, third person masculine singular

The root zxa means sieze, grasp. A n in front of a root can be one of two things:

בּ: in, with, through
particle: preposition

B; <  h; (the) +  B. (in)

בּ: in, with, through
particle: preposition

One of the functions of the preposition B. is to express means or instrument, so here it would mean by.

עלה: to go up
verb, hiphil, prefix, third person masculine singular with third person masculine singular suffix, vav-conv

In Genesis 22:2 you saw a Hifil imperative. Here you have a Hifil prefix with vav conversive. What happened to the characteristic h in front of the root marking the Hifil? Remember Genesis 22:3. The Hifil prefix does not have a preformative h but it does have two distinguishing characteristics.

1. The vowel patach under the prefix pronoun.

2. A “dot vowel” (hireq, sere, or seghol) under the second root letter.  

Note that the marks of vav conversive  ·w: are the same regardless of the stem of the verb. 

הוּא: he, she, it, himself, herself, the same, which (is), that (is),
pronoun, third person masculine singular

aWhh; is functioning as an attributive adjective modifyng ~AqM'h; and because they are both definite, they cause ~ve to be definite.

אֲשֶׁר: which, who
particle: relative

This word was introduced in Lesson 5 as the relative pronoun which or who. It can also open a dependent clause, as it does here, and mean that, when, because, where, as.

אמר: utter, say
verb, niphal, prefix, third person masculine singular

The root and prefix pronoun are familiar but the pointing is new. This is the 3 m. sg. Nifal prefix form. The Nifal affix form is distinctive because it has a preformative n as the name Nifal suggests. But the Nifal prefix does not begin with a preformative n

It is recognised by a characteristic vowel pattern.

Nifal prefix vowel pattern

For roots beginning with a guttural           XXeX' | Xe

For other roots                                        XXe X' | Xi (the bold X stands for a doubling dagesh) 

יוֹם: day
noun, masculine singular

Literally translated the day. This is often translated idiomatically today and in some translations even more freely until this day.

יהוה the LORD
proper name